Low-level Expression of Enzyme from Marine Bacillus Species for Detergents

Proteases are a varied and ancient family of enzymes that play a part in every aspect of an organism's functioning. As a member of the hydrolytic enzyme family, they help break down proteins into smaller peptides and amino acids. Proteases carry out a nucleophilic attack on the carbonyl carbon of the scissile bond to facilitate the addition of water. Because of the wide range of variances in their characteristics, such as substrate selectivity, active site composition, and catalytic mechanism, it is impossible to make generalizations regarding their stability and activity. Peptide synthesis, meat tenderization, cheese production, soy sauce production, silk industry organic syntheses, pharmaceutical industry waste treatment, protein hydrolyzation, and silver recovery from the waste photographic film are just some of the many applications of proteases, which have broad substrate specificity. Around 60% of all enzymes sold in the world are proteases derived from plants, animals, and microorganisms.